解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:Adverse events (AE) represent a significant clinical problem after infusion of cryopreserved HPC. However, the factors playing a role in the pathogenesis have not yet been fully established. METHODS:We prospectively collected data on AE that occurred with 179 HPC infusions performed on patients affected wit...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章
doi:10.1080/14653240701326756
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Tumor cell lines have enormous value for the study of different aspects of cancer biology and have also recently gained great importance in autologous cell-based anti-tumor therapies. However, the use of these cells is still limited because in vitro growth is hampered by suboptimal culture conditions and cur...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240701385836
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240701656053
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:The mechanism of platelet recovery after hematopoietic stem cell transplantation and the factors that influence its time-course are not fully understood. Rapid hematopoietic recovery results in a reduction of transplantation-related complications. In the present study, we questioned and analyzed whether ther...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240701561337
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Intracoronary application of BM-derived cells for the treatment of acute myocardial infarction (AMI) is currently being studied intensively. Simultaneously, strict legal requirements surround the production of cells for clinical studies. Thus good manufacturing practice (GMP)-compliant collection and prepara...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240601052734
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Post-infarct congestive heart failure is one of the leading causes of morbidity and mortality in industrialized countries. The main purpose of this study was to investigate whether transplantation of embryonic stem cell-derived cardiomyocytes (ESCM) directly into the infarcted myocardium could improve cardia...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240701247838
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Mesenchymal stromal cells (MSC) have the potential to differentiate into distinct mesenchymal tissues including cartilage, suggesting that these cells are an attractive cell source for cartilage tissue engineering approaches. Various methods, such as using hyaluronan-based materials, have been employed to im...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240600845070
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Multiple studies have demonstrated that 'purging' of autografts with 4-hydroperoxycyclophosphamide (4HC) or the related compound mafosfamide (Mf), to eradicate residual leukemia, produces the best results associated with autologous blood and marrow transplantation for AML. However, 4HC purging results in pro...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章
doi:10.1080/14653240600905353
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Adoptive transfer of autologous T cells that are gene-transduced to express Ag-specific receptors represents an experimental strategy to provide tumor-specific immunity to cancer patients. We studied this concept in patients with metastatic renal cell cancer (RCC) using retroviral transduction of T cells wit...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240601056396
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. METHODS:MSC were isolated from rhesus monkey BM and in...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240600760808
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Adipose tissue contains a stromal vascular fraction that can be easily isolated and provides a rich source of adipose tissue-derived mesenchymal stem cells (ASC). These ASC are a potential source of cells for tissue engineering. We studied whether the yield and growth characteristics of ASC were affected by ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240600621125
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Cells from AML patients can differentiate into the phenotype of DC when cultured with GM-CSF and IL-4. Such cytokine-treated AML-derived DC (AML-DC) can stimulate autologous T cells. In this study we examined whether an anti-CTLA-4 MAb (MDX-010) could enhance the generation of autologous anti-AML T cells. M...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240500499507
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:The use of myeloma Ag-loaded mature DC vaccines, cryopreserved in single-use aliquots, is an attractive immunotherapeutic strategy. In this study we investigated the retention of phenotype, viability and potency of DC vaccines after freezing and thawing. METHODS:Plastic-adherent monocytes, derived from a st...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240510027235
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process o...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240500361012
更新日期:2005-01-01 00:00:00
abstract::The efficacy of cord blood (CB) transplantation is limited by the low cell dose available. Low cell doses at transplant are correlated with delayed engraftment, prolonged neutropenia and thrombocytopenia and elevated risk of graft failure. To potentially improve the efficacy of CB transplantation, approaches have been...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240510027172
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:[corrected] Recent findings in cord blood (CB) research indicate the potential clinical usefulness of IL-2-activated CB in eradication of minimal malignant residual disease after hematopoietic stem cell transplantation. This feasible approach to immunotherapy merits further pre-clinical investigations using ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240500319184
更新日期:2005-01-01 00:00:00
abstract::Not until recently have Natural killer (NK) cells stepped out of the shadow of T-cells to be considered for cellular therapy of malignant diseases. This evolution has been facilitated by the discovery of specific receptors on NK cells that interact with HLA molecules on target cells but also the discovery of specific ...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240510018000
更新日期:2005-01-01 00:00:00
abstract::Despite improved prophylaxis and treatment, GvHD remains a major limitation to optimal allogeneic stem cell transplantation. Ex vivo selective depletion (SD) is a strategy to prevent GvHD, in which host-reactive donor lymphocytes are selectively eliminated from a PBSC allograft while useful donor immune function is pr...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240510018172
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:BM mesenchymal stem cells (MSC) have the capacity for renewal and the potential to differentiate into multiple tissues. In this study, we compared different enrichment methods to obtain MSC from BM. METHODS:Three different methods were compared with a view to obtaining MSC more rapidly from BM: negative sel...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240410004943
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Very few tumoral Ags have yet been isolated in NHL B cells. It is nevertheless possible to use whole tumor cells as a source of tumor Ags. We describe the purification of large numbers of human NHL B cells directly from lymph node or spleen biopsies, and different preparations allowing their use in a clinica...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240410006059
更新日期:2004-01-01 00:00:00
abstract::APC are used extensively to induce and expand Ag-specific T cells as well as to test their specificity and function. In the treatment of malignant and infectious diseases, APC are used to stimulate and expand Ag-specific T cells for adoptive transfer, or used directly in vivo to present Ag. The choice of APC to use de...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240410005230
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to investigate preservation of anti-leukemic activity and protection from opportunistic infections after transplantation of allogeneic + cells in patients with hematologic malignancies and bad prognosis. Methods Thirty-three patients [median age 42 years, range 23-55 years, diagnosi...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240410005375
更新日期:2004-01-01 00:00:00
abstract::Broader application of adoptive transfer of tumor-specific T-lymphocytes is accompanied by the need for effective suicide genes to ensure the safety of this cell-based therapy. In vivo elimination of T-lymphocytes expressing the herpes simplex virus-derived thymidine kinase gene has demonstrated the feasibility of thi...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240310001497
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Immunological and clinical effects of post-transplant growth factor administration have not been well studied. This report describes the outcome and immune functions of a total of 50 HLA-matched related donor allogeneic blood stem-cell transplantation patients who received post-transplant G-CSF (10 microg/kg...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/14653240310003648
更新日期:2003-01-01 00:00:00
abstract::More precise quantitation of cellular immune responses has become possible with the advent of single-cell assays of immune function, such as cytokine flow cytometry, enzyme-linked immunospot (ELISPOT), and MHC-peptide multimers. Cytokine flow cytometry is an attractive technique because it allows the detection of resp...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240310000029
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Hematopoietic progenitor stem cells (HPSC) are a specialized transfusion product used for transplantation. Microbial contamination may occur during harvest or subsequent manipulation of these cells. The same difficulties in ensuring a safe, sterile, final product are faced in the preparation of other cell-th...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240310003044
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:PBSC transplant provides 10 times more T cells than BMT However, the incidence and severity of acute GvHD is similar among recipients of both types of transplants. Studies in mouse models suggest that the similar clinical outcome in BMT and PBSCT is due to differences in the lymphokine profiles. METHODS:PBM...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240310002252
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Identification of HLA class I-restricted CMV epitopes, and the subsequent synthesis of HLA class I-peptide tetrameric complexes, have provided investigators with an important tool for visualising and quantifying the precise in vivo CTL response to CMV reactivation following stem cell transplantation. In conj...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532402317251518
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Traditionally, following high-dose therapy (HDT), unmanipulated autologous PBPC are infused. Alternatively, purified CD34+ cells can now be obtained by immunomagnetic separation using the CliniMACS device. Limited data currently exist examining hemopoietic recovery with such cells. METHODS:Ten patients with...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章
doi:10.1080/146532402317381857
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Container integrity is critical for maintaining sterility of cryopreserved cellular therapy products. We investigated a series of catastrophic bag failures, first noticed in early 2001. METHODS:Process records were reviewed for all PBPC and lymphocyte products cryopreserved in bags from January 2000 through...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532402761624700
更新日期:2002-01-01 00:00:00
abstract::Not all patients with Hodgkin's disease (HD) respond to standard chemotherapy and/or radiation, and hematopoietic stem-cell transplantation has been gaining increasing acceptance in the management of HD. Phase II and, to a lesser extent, phase III studies of high-dose chemotherapy and autologous stem cell transplantat...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/146532402320219754
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:When stem-cell grafts are infused into the venous circulation and stem/progenitor cells egress from BM, pulmonary capillary beds are the first microcirculation site that they encounter. This provides the potential for circulating progenitor cells to be trapped in the pulmonary circulation. METHODS:We compar...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532401317248063
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:CAMPATH-1 Abs have been used for T-cell depletion in stem-cell transplantation since the early 1980s. During that time there has been substantial progress in manufacturing techniques and quality control procedures. This article summarizes the methods used to produce the Abs for clinical use and describes res...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532401753174061
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:There is growing interest in the use of dendritic cells (DCs) for treatment of malignancy and infectious disease. Our goal was to develop a clinical scale method to prepare autologous DCs for cancer clinical trials. METHODS:PBMC were collected from normal donors or cancer patients by automated leukapheresis...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532401753156377
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:DS60 is a novel buoyant density solution, whose density has been adjusted to enrich PBSC from subjects who have been mobilized with cytokines alone, or cytokines plus chemotherapy. This report describes the use of BDS60 to enrich autologous PBSC that were used for hematological reconstitution after myeloabla...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章
doi:10.1080/146532400539134
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Ex vivo T-cell depletion of allogeneic BM (BM) grafts can effectively reduce graft versus host disease (GvHD) and may also apply to transplantation of allogeneic peripheral blood stem cell (PBSC) transplants. METHODS:Here we have evaluated T-cell depletion and progenitor cell recovery by antibody-mediated c...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532400539008
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Since 1978, 856 hematopoietic stem-cell transplantations have been performed for adult patients in Turkey. METHODS AND RESULTS:Data were collected on the source of hematopoietic progenitor cells, recipient diagnosis, conditioning regimen, DFS and mortality. The data were analyzed by the national registry sy...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532400539288
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Contaminating breast cancer cells in leukapheresis harvested for reinfusion to rebuild hemopoiesis after high-dose therapy have been described by several investigators. Methods for tumor cell detection are conventional immunocytochemistry, culture techniques and reverse transcriptase PCR. The percentage of t...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/0032472031000141251
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Mismatched family donor and unrelated donor BM transplants are associated with a high risk of acute GvHD. White T-cell depletion is the best method to reduce risk of acute GvHD, there was a reluctance to use T-cell depletion in the mismatched setting because of increased risk of rejection and relapse. Partia...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/0032472031000141284
更新日期:1999-01-01 00:00:00